Fluoro

iCheck Fluoro

iCheck Fluoro measures vitamin A in biological fluids and food

iCheck™ Fluoro is a state-of-the-art portable fluorometer. It determines vitamin A quantitatively in food and biological fluids. Using innovative technology it measures the fluorescence of vitamin A in the sample. The vitamin A concentration is displayed in μg RE/L (RE=retinol equivalent). It is used to measure vitamin A in vitamin premixes, sugar, flour, milk and biological fluids such as breast milk.

LOOK INSIDE
LOOK INSIDE

iCheck iLab Content

Stand for reagent vials

1x

User Manual and software on USB stick

1x

Battery charger and 4 rechargeable batteries

1x

User Manual and software on USB stick

1x

Digital balance and calibration weight

1x

Fluoro Standard

1x

iCheck Fluoro with metal cap

1x

iCheck Test Kit Content

Syringe

100x

Needle

100x

Vial

100x
Technical Data

Technical data

SAMPLE

Analyte
Vitamin A (retinol) as retinyl palmitate, retinyl acetate and other esters
Sample
Food: premix, liquid milk, milk powder, flour, sugar, bouillon powder; biological fluids: breast milk, whole blood, serum
Sample preparation
For solid samples: dilution and homogenization is distilled or bottled water
Sample volume per analysis
0.5 mL (500 μL)
Concentration range
>0.05 ppm (mg/kg), samples above 3.0 ppm must be diluted in water

DEVICE

Analytical method
Fluorimetric determination of retinol concentration using ultraviolate (UV) excitation
Units displayed
μg RE/L, RE – retinol equivalents, μg – microgramms
Linear range
50–3000 μg RE/L
Calibration
Factory set (standards included for control)
Time per analysis
< 10 min
Enviroment
20-30°C, no direct sunlight
Accuracy at 95% confidence interval at 25°C
±5-25% (depends on the sample type)
Method comparison
High-performance liquid chromatography (HPLC)
User training
1 day training
Use
Laboratory and field
Data output
Sample #, Batch #, Result, Date, Time (in transferred data)
Connectivity and data
Results are stored in the device and transferred to a PC via USB
Power source
NiMH rechargeable batteries included; AA 1.2 or 1.5V
Warranty
2 years
Device weight
0.45 kg
Device dimensions
11 x 4 x 20 cm (W x H x L)

TEST KIT

Content
100 reagent vials; 100 syringes – 1.0 mL; 100 needles – 1.6mm x 25mm
Chemical composition
n-Hexan and alcohols
Volume per reagent vial
2.0 mL
Shelf life
12 months at 20-30°C, no direct sunlight, upright
Dimension of test kit
26 x 14.5 x 16.5 cm
Disposal instructions
Hazardous waste
Optional equipment 
Manual centrifuge, 50 mL falcons, weighing dishes, reference samples 
How it works

How it works

Reference Methods

iCheck Fluoro is comparable
to reference methods

LEARN MORE
Application Notes

Application Notes

Vitamin A in Rice

Vitamin A in Milk and Milk Powders

Scientific Publications

Scientific Publications

1. Laillou, A. Assessment of a portable device to quantify vitamin A in fortified foods (flour, sugar, and milk) for quality control. Food Nutr Bull. 2014

2. Engle-Stone, R. Comparison of breast milk vitamin A concentration measured in fresh milk by a rapid field assay (the iCheck FLUORO) with standard measurement of stored milk by HPLC. Eur J Clin Nutr. 2014

3. DeJaeger, A. Food fortification and vitamin A analysis. Food, Science and Technology Magazine, 2014

4. Zambo, Z. Mounting and Adaptation of a Fluorescent Rapid-Assay Device (iCheck™ FLUORO) for Vitamin A in Sugar and Biological Fluids. Sight and Life Magazine , Vol. 27, 2013

5. Bechir, M. Retinol Assessment Among Women and Children in Sahelian Mobile Pastoralists. EcoHealth, 2012

6. Dary,O. A New Test Kit’s Potential for the Rapid Analysis of Vitamin A in Human and Cow Milk. Sight and Life Magazine , Vol. 25, 2011

Material Data Safety Sheets

Material Data Safety Sheets

Looking for the User Manual?
Need Help with Sample Preparation?
Find it all on iCheck Academy

iCheck Academy